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total igf- 1  (ALPCO)


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    ALPCO total igf- 1
    Total Igf 1, supplied by ALPCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total igf- 1/product/ALPCO
    Average 90 stars, based on 1 article reviews
    total igf- 1 - by Bioz Stars, 2026-02
    90/100 stars

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    Comparison of sex, age, fructosamine concentration and insulin dose between diabetic cats with and without increased IGF-1 (IGF-1 ⩽746 ng/ml) suspicious for hypersomatotropism

    Journal: Journal of Feline Medicine and Surgery

    Article Title: Signalment, clinicopathological findings, management practices and comorbidities in cats with diabetes mellitus in Germany: cross-sectional study of 144 cases

    doi: 10.1177/1098612X241303303

    Figure Lengend Snippet: Comparison of sex, age, fructosamine concentration and insulin dose between diabetic cats with and without increased IGF-1 (IGF-1 ⩽746 ng/ml) suspicious for hypersomatotropism

    Article Snippet: Veterinary practices and owners of diabetic cats could participate if they submitted a completed questionnaire (see Appendix 1 in the supplementary material ) alongside blood samples for haematology (ADVIA 2120i; Siemens Diagnostics), serum biochemistry, DGGR ester lipase activity, concentration of fructosamine, b-hydroxybutyrate (BHB) (all measured by AU 5800, AU 680; Beckman Coulter and Alinity CI-Systems), total thyroxine (TT4), COB and IGF-1 (all three measured using IMMULITE 2000 XPi Immunoassay System; Siemens Medical Solutions Diagnostics).

    Techniques: Comparison, Concentration Assay

    Silencing of InsR disrupted InsR/IGF-1R hybrid receptor formation and increased IGF-1R homodimer formation. ( A ) Lysates from non-transfected MES-13 mesangial cells were immunoprecipitaed (IP) using antibodies against IGF-1Rα, InsRα or control (CTR) IgG, an irrelevant control antibody. Immunoprecipitates were immuneblotted (IB) for IGF-1Rβ or InsRβ. WCL, whole cell lysate. An image of gel stained after transfer was shown as a loading monitor (lower panel). ( B ) Hybrid receptors in SC or InsR shRNA (sh-InsR) transfected cells were detected by immunoprecipitation with antibodies directed against either the InsRα or IGF-1Rα. Immunoprecipitated receptors were detected by immunoblotting with an anti-IGF-1Rβ antibody (upper panel). An image of gel stained after transfer was shown as a loading monitor. Immunodepletion was assessed by immunoblotting the supernatants from immunoprecipitate with an anti-IGF-1Rβ antibody (middle panel). The bar graph shows the densitometric scanning results (upper panel, means ± SEM) of four independents experiments *, P <0.05.

    Journal: Cell Communication and Signaling : CCS

    Article Title: In vitro silencing of the insulin receptor attenuates cellular accumulation of fibronectin in renal mesangial cells

    doi: 10.1186/1478-811X-10-29

    Figure Lengend Snippet: Silencing of InsR disrupted InsR/IGF-1R hybrid receptor formation and increased IGF-1R homodimer formation. ( A ) Lysates from non-transfected MES-13 mesangial cells were immunoprecipitaed (IP) using antibodies against IGF-1Rα, InsRα or control (CTR) IgG, an irrelevant control antibody. Immunoprecipitates were immuneblotted (IB) for IGF-1Rβ or InsRβ. WCL, whole cell lysate. An image of gel stained after transfer was shown as a loading monitor (lower panel). ( B ) Hybrid receptors in SC or InsR shRNA (sh-InsR) transfected cells were detected by immunoprecipitation with antibodies directed against either the InsRα or IGF-1Rα. Immunoprecipitated receptors were detected by immunoblotting with an anti-IGF-1Rβ antibody (upper panel). An image of gel stained after transfer was shown as a loading monitor. Immunodepletion was assessed by immunoblotting the supernatants from immunoprecipitate with an anti-IGF-1Rβ antibody (middle panel). The bar graph shows the densitometric scanning results (upper panel, means ± SEM) of four independents experiments *, P <0.05.

    Article Snippet: Antibodies against InsRα, InsRβ, IGF-1Rα and phospholylated or total CREB-1 were purchased from Santa Cruz Biotechnology.

    Techniques: Transfection, Staining, shRNA, Immunoprecipitation, Western Blot